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SUMMARY:Introduction to Next Generation Sequencing
DTSTART;VALUE=DATE-TIME:20240522T083000Z
DTEND;VALUE=DATE-TIME:20240522T103000Z
DTSTAMP;VALUE=DATE-TIME:20260429T060728Z
UID:indico-contribution-487-387@indico.giga.uliege.be
DESCRIPTION:Speakers: Arnaud Lavergne (GIGA)\nNext-Generation Sequencing (
 NGS)\, also called High-Throughput Sequencing (HTS)\, is a technology allo
 wing the massively parallel processing of millions of sequences per run.\n
 \nDuring this lecture (~2hrs)\, we will discuss both the theorical and pra
 ctical aspects behind this technology. Sample selection\, RNA/DNA extracti
 on and library preparation are common steps for different applications of 
 NGS/HTS. We will discuss data analysis\, which will be slightly different 
 from an application to another one\, while some important steps are simila
 r and fundamental in most NGS/HTS applications. Finally\, we will have a f
 irst insight into the concept of pipeline and analysis reproductibility.\n
 \nAt the end of the lecture\, participants will be familiar with NGS/HTS t
 echnology and the different steps associated with a NGS/HTS experiment. Th
 ey will also understand what are the key elements to consider when startin
 g a NGS/HTS project.\n\nhttps://indico.giga.uliege.be/event/487/contributi
 ons/387/
LOCATION:GIGA meeting rooms B34/+5-42 - Grande Ghuysen
URL:https://indico.giga.uliege.be/event/487/contributions/387/
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BEGIN:VEVENT
SUMMARY:Introduction to Transcriptomics
DTSTART;VALUE=DATE-TIME:20240522T113000Z
DTEND;VALUE=DATE-TIME:20240522T143000Z
DTSTAMP;VALUE=DATE-TIME:20260429T060728Z
UID:indico-contribution-487-386@indico.giga.uliege.be
DESCRIPTION:Speakers: Arnaud Lavergne (GIGA)\nRNA Sequencing (RNASeq) is o
 ne of the most famous applications of NGS/HTS technology. It allows the st
 udy of gene expression at the genome-wide level.\n\nDuring this lecture\, 
 we will discuss both theorical and practical aspects underlying the classi
 cal RNASeq process. We will also cover some variations of the protocol and
  different alternative methods. Moreover we will explore important steps i
 n a RNAseq data analysis and describe the input/output files involved.\n\n
 Single-Cell (SC) sequencing allows to study sequencing information at the 
 level of an individual cell. After the individualization of your cells.\n\
 nWe will discuss both theorical and practical aspects underlying the SC se
 quencing process. We will then discuss further the technology in the conte
 xt of single-cell RNA sequencing (scRNAseq) and how it differs from "bulk"
  RNAseq data in terms of data analysis.\n\nAt the end of the lecture\, par
 ticipants will be familiar with sequencing technology and its applications
  in the context of Transcriptomics studies. They will be able to explain t
 he key elements/steps to consider in a bulk/ScRNAseq project.\n\nhttps://i
 ndico.giga.uliege.be/event/487/contributions/386/
LOCATION:GIGA meeting rooms B34/+5-42 - Grande Ghuysen
URL:https://indico.giga.uliege.be/event/487/contributions/386/
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