Hands-on - Transcriptomics & Bioinformatics analysis (BIF-TBA0001)

Wednesday 27 Mar 2024, 10:30 → 17:00 Europe/Brussels

GIGA meeting rooms

Arnaud Lavergne (GIGA)

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This hands-on follows the "NGS & Transcriptomics" lecture.

Following the lecture, the aim of this training is to give to the participants the basis to perform their own Transcriptomics downstream analysis.

After a short introduction, participants would be able to choose between bulkRNAseq, scRNAseq or both analysis.

BulkRNAseq - Using DESeq2 standard workflow, you will first learn to conduct an analysis for a simple design. We will go through the different steps including establishing the design, normalization, clustering and differential expression analysis.

ScRNAseq - Using Seurat standard workflow, you will first learn to conduct a simple SC analysis on 10X genomics data. We will go through the different steps including integrating the data, filtering, normalization, clustering and differential expression analysis.

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Requirements :

- You attended the "Introduction to NGS & Transcriptomics" lecture (optional, but no reminder during hands-on)

- You attended the "R Introduction" trainings (OR proof of R knowledge)

- You will need your own computer with R and/or Rstudio installed

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Organization :

1-Day Session

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Price (HTVA):

- GIGA/Uliege : 100€

- CHU : 115€

- Academic non-Uliege : 140€

- Company/Industry: 200€

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10:30 → 12:00 Introduction to Transcriptomics Analysis

During this session, we will have an overview of Transcriptomics analysis and we will discuss the different steps of classical downstream analysis
https://dox.ulg.ac.be/index.php/s/UvZ0xwUZTGPiXYF.

Speaker: Dr Arnaud Lavergne (GIGA)

13:00 → 17:00 Downstream Analysis - Hands-on

Hands-on session where the participants will have to perform donwstream analysis of Transcriptomics data.

Speaker: Dr Arnaud Lavergne (GIGA)