Next-Generation Sequencing (NGS), also called High-Throughput Sequencing (HTS), is a technology allowing the massively parallel processing of millions of sequences per run.
During this lecture (~2hrs), we will discuss both the theorical and practical aspects behind this technology. Sample selection, RNA/DNA extraction and library preparation are common steps for different applications of...
RNA Sequencing (RNASeq) is one of the most famous applications of NGS/HTS technology. It allows the study of gene expression at the genome-wide level.
During this lecture, we will discuss both theorical and practical aspects underlying the classical RNASeq process. We will also cover some variations of the protocol and different alternative methods. Moreover we will explore important steps...
Single-Cell (SC) sequencing allows to study sequencing information at the level of an individual cell. After the individualization of your cells, you can apply your favorite NGS technology to your sample.
During this lecture, we will discuss both theorical and practical aspects underlying the SC sequencing process. We will then discuss further the technology in the context of single-cell...