Hands-on - Single-Cell RNAseq analysis using Seurat

18 Apr 2023, 14:00 → 25 Apr 2023, 16:30 Europe/Brussels

GIGA meeting rooms

Arnaud Lavergne (GIGA)

---

This hands-on follows the "Introduction to Single-Cell Sequencing" lecture.

Following the introduction, the aim of this training is to give to the participants the basis to perform their own Single-Cell RNAseq downstream analysis.

Using Seurat standard workflow, you will first learn to conduct a simple SC analysis on 10X genomics data. We will go through the different steps including integrating the data, filtering, normalization, clustering and differential expression analysis.

In the second session, you will be able to choose to perform an analysis on your own data, or provided data with a more complex design.

---

Requirements :

- You attended the "Introduction to Modern Sequencing" lecture

- You attended the "R Introduction" trainings (OR proof of R knowledge)

- You will need your own computer with R and/or Rstudio installed

---

Organization :

2 Sessions ~3Hrs

---

Price :

- GIGA/Uliege : 30€

- Academic non-GIGA/Uliege : 40€

- Industry : 75€

---

 

Tuesday, 18 April

14:00 → 16:30 Seurat - Exploring vignettes for standard analysis

During this session, we will apply the Seurat workflow on a 10X genomics dataset, and we will discuss the different steps and what they imply.
We will cover data import, filtering & QC, normalization, clustering and visualization.

Speaker: Dr Arnaud Lavergne (GIGA)

Data.zip

SCRNASEQ ANALYSIS USING SEURAT.pdf

Tuesday, 25 April

14:00 → 16:30 Seurat - Your scRNAseq analysis

During this session, participants will perform a complete Seurat workflow on a dataset.
They will be able to choose between their own data, or provided datasets.

Speaker: Dr Arnaud Lavergne (GIGA)

Integration_P2.R

Integration.R