Hands-on - RNAseq analysis using DESeq2

30 Mar 2023, 13:30 → 6 Apr 2023, 16:00 Europe/Brussels

GIGA meeting rooms

Arnaud Lavergne (GIGA)

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This hands-on follows the "Introduction to RNA Sequencing" lecture.

Following the introduction, the aim of this training is to give to the participants the basis to perform their own RNAseq downstream analysis.

Using DESeq2 standard workflow, you will first learn to conduct an analysis for a simple design. We will go through the different steps including establishing the design, normalization, clustering and differential expression analysis.

In the second session, you will be able to choose to perform an analysis on your own data, or provided data with a more complex design.

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Requirements :

- You attended the "Introduction to Modern Sequencing" lecture

- You attended the "R Introduction" trainings (OR proof of R knowledge)

- You will need your own computer with R and/or Rstudio installed

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Organization :

2 Sessions ~3Hrs

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Price :

- GIGA/Uliege : 30€

- Academic non-GIGA/Uliege : 40€

- Industry : 75€

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Thursday, 30 March

13:30 → 16:00 DESeq2 standard workflow

The session will cover the complete analysis of a simple RNAseq experiment. We will describe each step and what it implies during the processing.

Speaker: Dr Arnaud Lavergne (GIGA)

transcriptomics-main.zip

Thursday, 6 April

13:30 → 16:00 DESeq2 - Adapting workflow to datasets

It this session, you will focus on how to configure DESeq2 worklow to fit with a specific dataset. Participants will be able to analyze their own datasets, or some provided datasets.

Speaker: Dr Arnaud Lavergne (GIGA)

Scripts_Dataset_A.R